Journal: Regenerative Therapy

Article Title: Orexin-A increases the differentiation of human olfactory sensory neurons through orexin receptor type 1

doi: 10.1016/j.reth.2024.10.014

Figure Lengend Snippet: Schematic diagram for primary HONCs culture establishment. (a) An excised specimen from human olfactory neuroepithelium. (b) Immunohistochemical stain with OMP (green), CK5 (red), and DAPI (blue) represents mature OSNs, basal cells, and nuclei, respectively (top). β4-tubulin (red) and DAPI (blue) immunohistochemical markers were to rule out respiratory epithelium (scale bar = 100 μm). (c) Schematic showing HONCs maturation process from progenitor cells to mature neuronal cells. (d) Time course of the experimental design with accompanying microscopy indicating cell migration from explants (scale bar = 1 mm).

Article Snippet: Sections were washed with PBS, and then mounted with FluoroshieldTM with DAPI (GTX30920, Genetex, CA, USA) for further observation.

Techniques: Immunohistochemical staining, Staining, Microscopy, Migration

Journal: Regenerative Therapy

Article Title: Orexin-A increases the differentiation of human olfactory sensory neurons through orexin receptor type 1

doi: 10.1016/j.reth.2024.10.014

Figure Lengend Snippet: Immunofluorescence of signal transduction protein for cultures with or without orexin-A (1 ng/mL). The cells stained with protein marker (green), (a) ADCY3 and (b) Golf protein markers are used to identify olfactory signal transduction. Cellular cytoskeletal shape (F-actin) and nuclei are visualized with staining using phalloidin-ifluor 555 (in red) and DAPI (in blue), respectively (scale bar = 100 μm).

Article Snippet: Sections were washed with PBS, and then mounted with FluoroshieldTM with DAPI (GTX30920, Genetex, CA, USA) for further observation.

Techniques: Immunofluorescence, Transduction, Staining, Marker